新型纳米抗菌无机填料的合成及其抗菌性能的初步研究

[摘要] 目的 合成新型的纳米抗菌无机填料，为赋予牙科复合树脂抗菌性能提供新的思路。方法 通过有机合成的方法，首先合成了季铵盐：N,N,N-三甲基-3-（三甲氧基硅基）丙基-1-碘化铵，然后再将其枝接到纳米二氧化硅颗粒表面，得到新型纳米抗菌无机填料季铵盐修饰纳米二氧化硅，并对合成产物结构进行表征。然后以变异链球菌为对象，研究季铵盐修饰纳米二氧化硅的抗菌性能。结果 红外光谱分析表明，季铵盐成功地枝接到了纳米二氧化硅颗粒表面。与对照组相比，新型纳米抗菌无机填料对变异链球菌具有较强的杀菌作用（P

铵盐修饰纳米二氧化硅颗粒具有较好的抗菌性能，可以用来提高牙用复合树脂的抗菌性。

[关键词] 复合树脂； 抗菌性； 无机填料； 季铵盐

[中图分类号] R 783.1 [文献标志码] A [doi] 10.3969/j.issn.1000-1182.2012.05.018

Study on synthesis on a novel nano-antibacterial inorganic filler and its antibacterial property Wu Junling1, Jin Chanyuan1, Nie Xiaomeng1, Zhou Kaiyun2, Zhou Chuanjian2. （1. Dept. of Prosthodontics, College of Stomatology, Shan-dong University; Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China; 2. Research Institute of Polymer Materials, School of Materials Science and Engineering, Shandong University, Jinan 250061, China）

[Abstract] Objective To synthesize a novel nano-antibacterial inorganic filler and provide a new way to give dental composite resin antibacterial property. Methods Quaternary ammonium iodide N,N,N-trimethyl-3-（trimethoxysilyl） propan-1-aminium iodide were organically synthesized firstly and then the N,N,N-trimethyl-3-（trimethoxysilyl） propan-1-aminium iodide was grafted to the nano-silica particle to synthesize the antibacterial inorganic fillers nano-silica particle grafted with quaternary ammonium salt. All the products were analyzed and identified by infrared spectrum analysis. Then Streptococcus mutans were chosen as experimental object to analysis the antibacterial property of nano-antibacterial inorganic filler. Results Quaternary ammonium salt was grafted to the surface of nano-silica particles successfully by infrared spectrum analysis. Compared with the control group, the nano-silica particle grafted with qua-ternary ammonium salt had a strong bactericidal effect on Streptococcus mutans（P

particle grafted with quaternary ammonium salt has a strong antibacterial property and could be used to improve dental composite resin antibacterial property.

[Key words] composite resin； antibacterial property； inorganic filler； quaternary ammonium salt

现有的绝大多数牙科复合树脂材料因没有抗菌性能，容易造成充填修复后患牙的继发龋、牙髓炎症等并发症，如何赋予复合树脂材料抗菌、防龋性能，有效抑制微生物、菌斑在其表面及周围的滋生和附着，提高临床治疗效果，是牙科材料的研究热点[1]。为了实现上述目的，研究者从不同方面进行了

尝试，如通过直接向树脂基体中加入抗菌成分，或通过在树脂基质上键合抗菌官能团等方法，来抑制细菌滋生等[2-3]。这些方法虽取得了一定的效果，但如何进一步提高树脂的抗菌性能依然是摆在研究者面前的一个课题。季铵盐作为一类广谱抗菌化合物，在抗菌领域具有广泛的应用，将高分子季铵盐与无机颗粒复合可以制成非缓释型抗菌材料[4]。如果将具