逆针灸关元对去卵巢大鼠下丘脑-垂体-卵巢轴影响

摘要：目的 观察逆针灸“关元”对去卵巢大鼠的影响,探讨其调节围绝经期综合征下丘脑-垂体-卵巢(HPO)轴的机制。方法 将3月龄SD雌性大鼠48只随机分为正常组、假手术组、去卵巢组、逆针关元组、逆灸关元组。术前30 d给逆针关元组和逆灸关元组予以针刺或艾灸,随后制作去卵巢大鼠模型,除正常组和假手术组外,其余组均行去卵巢手术。采用酶联免疫法测定大鼠下丘脑、垂体及子宫的促性腺激素释放激素(GnRH)、卵泡刺激素(FSH)、促黄体生成激素(LH)、雌二醇(E2)、雌激素受体(ER)的水平变化。结果 与正常组比较,模型组E2、ER和GnRH水平显著降低(P＜0.01),FSH和LH的水平显著升高(P＜0.01)。与模型组比较,逆针和逆灸组E2、ER和GnRH水平均不同程度升高(P＜0.05),FSH和LH水平不同程度降低(P＜0.05)。逆针组和逆灸组比较,组间差异无统计学意义(P＞0.05)。结论 逆针和逆灸“关元”均能良性地调节去卵巢大鼠HPO轴的紊乱,其对HPO轴的作用强度基本相同。

关键词：关元；针灸；下丘脑-垂体-卵巢轴；围绝经期综合征

DOI：10.3969/j.issn.1005-5304.2013.08.016

中图分类号：R245

文献标识码：A

文章编号：1005-5304(2013)08-0045-03Effects of Preventive-electroacupuncture and Preventive-moxibustion at Guanyuan (RN4) on Hypothalamus-Pituitary-Ovary Axis in Ovariectomized Rats LI Na, CHENG Kai, ZHOU Jing (School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China)

Abstract：Objective To observe the regulatory effects of the preventive acupuncture and moxibustion at Guanyuan (RN4) points in ovariectomized rats, and discuss the mechanism of preventive acupuncture and moxibustion on hyoathalamus-pituitary-ovary (HPO) axis. Methods Totally 48 female SD rats were divided into 5 groups, including normal group, sham operation group, ovariectomized rats group, preventive acupuncture group and preventive moxibustion group. Except normal group and sham operation group, rats were ovariectomized after preventive acupuncture and preventive moxibustion for 30 d in corresponding groups. ELISA was used to determine the levels of E2, ER, GnRH, FSH and LH in the tissues including hypophysis, hypothalamus and uterus. Results Compared with the normal group, E2, ER and GnRH level in the tissue of the model group was significantly lower (P＜0.01), the levels of FSH and LH increased obviously (P＜0.01). Compared with the model group, E2, ER and GnRH level of preventive acupuncture group and preventive moxibustion group increased in different degree (P＜0.05). FSH and LH level reduced in different degree (P＜0.05). There was no obvious difference between preventive acupuncture and preventive moxibustion group (P＞0.05). Conclusions The preventive acupuncture and preventive moxibustion at Guanyuan (RN4) has benign modulation to disordered HPO axis of ovariectomized rats. The ability of preventive acupuncture and preventive moxibustion are almost the same.

Key words：Guanyuan；acupuncture and maxibustion；hypoathalamus-pituitary-ovary axis；menopansal syndrome

围绝经期综合征是指因雌激素水平波动或下降所致的以植物性神经系统功能紊乱合并神经心理症状为主的综合征,多发生于45～55岁之间[1]。由于卵巢功能退行性改变,导致下丘脑-垂体-卵巢(HPO)轴功能失调,卵巢停止排卵,垂体分泌的卵泡刺激素增高,雌激素缺乏,内分泌、代谢、神经、心理等各系统失衡而引起的一组症候群。西医一般采用雌激素替代疗法,尽管疗效肯定,但存在适应症及禁忌症,甚至会诱发乳腺癌与子宫内膜癌。近年来,针灸因其疗效确切,作用持久、无不良反基金项目：教育部新世纪优秀人才支持计划(0100413)通讯作者：程凯,E-mail：应特点而被广泛接受。逆针灸是指在疾病发生前,预先用针灸激发经络之气,扶助正气,提高机体抵御疾病的能力,从而预防疾病的发生、减轻疾病的损害,达到保健延年的目的,是一种注重机体自身潜能的激发与利用的方法,对神经-内分泌-免疫系统有重要的调节作用[2-4]。本实验采用逆针灸的方法,观察其对随后去卵巢大鼠雌激素水平的影响。1 实验材料1.1 动物

健康SD雌性大鼠,3月龄,清洁级,48只,体质量(220±20)g,北京维通利华实验动物技术有限公司提供,动物许可证号：SCXK(京)2006-0008。喂养于湿度65%、温度(20±2)℃、12 h光暗周期的条件下。