单核细胞趋化蛋白-1基因A-2518G多态性与急性冠脉综合征相关性研究

【摘要】目的 探讨中国苏南地区汉族人群单核细胞趋化蛋白-1（MCP-1）基因启动子区A-2518G 单核苷酸多态性与 ACS 发病的相关性。方法 采用病例-对照研究方法，选择临床确诊的ACS 484 例（ACS组），其中急性心肌梗死（AMI）290 例，不稳定性心绞痛（UAP）194 例。经冠脉造影排除冠心病者 346 例为对照组，包括冠脉硬化症（CAS 组）166 例和冠脉无狭窄（冠脉正常组）180 例。利用聚合酶链式反应-限制性酶切片段长度多态性技术（PCR-RFLP）检测MCP-1基因 A-2518G 多态性。结果 MCP-1 基因 A-2518G 单核苷酸多态性在 ACS 组和对照组中均存在 AA、AG 和 GG 三种基因型。二组基因型分布符合 Hardy- Weinberg 平衡（P＞0.05），具有群体代表性。与对照组相比，ACS 组中 AA（15.32% vs. 16.12%）、AG（53.47% vs. 51.86%）和 GG（31.21% vs. 32.02%）基因型和 G（57.95% vs. 57.95%）等位基因频率差异均无统计学意义（P值分别为0.083、0.673、0.821 和 1.000）。对性别、年龄、吸烟、糖尿病、TC 和 LDL 等 6 个相关因素行 Logistic 回归分析显示，MCP-1 基因 A-2518G 单核苷酸多态性与ACS 的发病无相关性（P>0.05）。对男性ACS、女性 ACS、AMI、UAP 和早发 ACS 等 5 个亚组进行 MCP-1 基因 A-2518G 多态性分析，结果显示，男性和女性 ACS 分别与对照组相比、AMI 和 UAP 分别与冠脉正常组相比以及早发 ACS 与年龄相匹配的对照组相比，AA、AG 和 GG 基因型和 G 等位基因频率差异均无统计学意义（均P>0.05）。结论 在中国苏南地区汉族人群中，MCP-1 基因存在 A-2518G 单核苷酸多态性，该多态性与 ACS 发病无显著相关性。

【关键词】单核细胞趋化蛋白-1；单核苷酸多态性；急性冠脉综合征

Correlation between polymorphism of monocyte chemoatt-ractant protein-1 gene A-2518G single nucleotide and acute coronary syndrome SHI Gan-wei，HE Guo-ping，CAI Gao-jun，QI Chuan-ping，GAO Lei，QI Meng，SHENG Dan-dan，QIAN Zhi-hong，XU Lian-hong.省略

【Abstract】Objective To investigate the possible correlation between the monocyte chemoattractant protein-1 (MCP-1) gene A-2518G single nucleotide polymorphism (SNPs) in the promoter region and acute coronary syndrome (ACS) in Chinese Han ethnic population of Sunan region.Methods This study was conducted with a case-control design in 484 ACS patients including 290 acute myocardial infarction (AMI) patients and 194 patients with unstable angina pectoris (UAP) and 346 control subjects ruled out coronary disease by coronary angiography (control group)，including 166 patients with coronary atherosclerosis and 180 subjects without coronary stenosis. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for the detection of the A-2518G polymorphism in MCP-1 gene，and then the frequency of genetype was statistically analyzed. Results There were AA, AG and GG genotypes of MCP-1 gene A-2518G polymorphism in the ACS group and control group. The two groups could be considered as a genetic equilibrium representative by Hardy-Weinberg equilibrium (P>0.05). Compared with the control group, the frequencies of AA genotype (15.32% vs. 16.12%), AG genotype (53.47% vs. 51.86%), GG genotype (31.21% vs. 32.02%) and G allele genotype (57.95% vs. 57.95%) in ACS group were not significantly different (P was 0.083, 0.673, 0.821 and 1.00, respectively). Multivariate logistic regression analysis indicated that there was no significant correlation between MCP-1 gene A-2518G polymorphism and ACS regardless of differences in gender, age, smoking, diabetes, TG and LDL-C (P>0.05). There was no significant difference in gender and age of ACS onset between two groups (P>0.05). There were no significant differences in the frequencies of AA, AG and GG genotypes and G allele genotype among AMI group, UAP group and normal coronary group (P>0.05). Conclusions The data shows that MCP-1 gene A-2518G polymorphism is not associated with the risk of ACS in the Chinese Han ethnic population living in Sunan region.