吸烟和细胞色素P450 1A1-MspⅠ、谷胱甘肽硫转移酶T1基因多态性与口腔癌的相关性研究

[摘要] 目的 探讨吸烟和细胞色素P450（CYP）1A1-MspⅠ、谷胱甘肽硫转移酶（GST）T1基因多态性与口腔癌发病之间的关系。方法 采用病例-对照研究的方法，以300例口腔癌患者（病例组）及300例非癌对照者（对照组）的外周血白细胞为样本，利用聚合酶链反应（PCR）技术检测Ⅰ相代谢酶CYP1A1-MspⅠ和Ⅱ相代谢酶GSTT1基因多态性，并分析吸烟和2种代谢酶基因多态性与口腔癌的相关性。结果 病例组CYP1A1-MspⅠ突变纯合型（m2/m2）和GSTT1基因缺陷型[GSTT1（-）]的频率分布分别为38.33%、69.33%，而对照组的频率分布分别为21.00%、44.33%，二者差异有统计学意义（P

[关键词] 口腔癌； 细胞色素P450 1A1-MspⅠ； 谷胱甘肽硫转移酶T1； 多态现象； 吸烟

[中图分类号] R 739.8 [文献标志码] A [doi] 10.3969/j.issn.1000-1182.2012.02.019

Correlation between smoking and the polymorphisms of cytochrome P450 1A1-MspⅠand glutathione S-trans-ferase T1 genes and oral cancer Guo Like1, Zhang Chaoxian2, Shi Shumin1, Guo Xiaofeng3. （1. Dept. of Stomato-logy, The First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, China; 2. Dept. of Gastroenterology, The First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, China; 3. Dept. of Hepatology, The Sixth People’s Hospital of Hangzhou, Hangzhou 310014, China）

[Abstract] Objective To investigate the correlation between the combination of smoking and the polymorphisms

of cytochrome P450（CYP） 1A1-MspⅠ and glutathione S-transferase（GST） T1 genes and oral cancer. Methods The genetic polymorphisms of CYP1A1-MspⅠ and GSTT1 were detected by polymerase chain reaction（PCR） technique

in peripheral blood leukocytes of 300 oral cancer cases and 300 non-cancer controls, and the correlation between smo-king, the two metabolic enzymes genetic polymorphisms and oral cancer were analyzed. Results The frequencies of CYP1A1-MspⅠ（m2/m2） and GSTT1（-） were 38.33% and 69.33% in oral cancer cases and 21.00% and 44.33% in healthy controls respectively. Statistical tests showed significant difference in the frequencies between the two groups

（P

2.34, 95%CI 1.76-4.07）. The individuals who carried with GSTT1（-） had a high risk of oral cancer（OR=2.84, 95%CI 1.98-4.54）. Combined analysis of the polymorphisms showed that percentage of CYP1A1-MspⅠ（m2/m2)/GSTT1（-）