The Study on Specific Enzymes in Actinidia Arguta

Abstract: Actinidia Arguta fruit postharvest softening two phases, the first stage softening faster, plays a main role with the phases of amylase enzymes are 7 the first Second stage softening slowly, the main effect since the phases of enzymes are together with more than half of lactose hyaluronic acid enzymes. Ethylene release to promote the role of fruit softening protective enzyme peroxidase, catalane appeared in late fruit softening, so not fruit softening the phases of enzyme specificity.

Key words: Specific enzymes; actinidia arguta; Fruit Softening

1 Introduction

Kiwi (Actinidia SPP.) is introduced to the New Zealand from their origin in China (sir alex ferguson 1984) and then as a successful fruit crops developed world trade in the twentieth century. Actinidia Arguta (green to kiwi) and a (yellow kiwi) are two kiwi kind of commercial cultivated varieties. Recently, a new cultivation, Actinidia arguta, has become a commercial of kiwi fruit-producing several countries, including New Zealand. Fruit Actinidia arguta thankful for their rich flavor consumers, especially for their small grape size and smooth, glabrous, edible skin (Matich et al. 2003; Williams et al. 2003). Actinidia of a wide range of species diversity and a rich genetic resources, it provides great continues to improve and enhance the sustainability of potential varieties of kiwi fruit industry of the world. Although all business kiwi fruit varieties can use at present traditional breeding has developed technology (MaiKeLei 2007), considerable progress to promote the adoption of the breeding programmes kiwi a series of molecular tools and biotechnology. Recently, a large data sets 132577 Actinidia said sequence tags (est), mainly from four Actinidia species (Actinidia prawns, a, a, a deliciosa arguta eriantha) have been released (LaoHe et al. 2008). Genomics method, the function of the gene that system functional genomics methods in model plant arabidopisis thaliana, etc, the use of Actinidia gene three varieties of improvement, has become a very attractive prospect (Wang and Lin-Wang 2007).

Gene function analysis model plant species some limitations especially if model lack of specific biochemical pathway or development process of candidate genes are expected to participate. This requirement, clarify this kind of the function of the gene, conversion system are interested in plant species must establish transformants generate stable in a highly efficient, reliable and renewable method. Agriculture conversion is a perfect methods for transferring specific gene into a wide range of species of plants. Kiwi fruit varieties conversion agreement has already Actinidia deliciosa report (Jason and Gardner 1993; Matsuta et al. 1990; meeting et al. 1991), Actinidia shrimp (Fraser et al. 1995; and the king, et al. 2007) and Actinidia eriantha (king et al. 2006). The root agrobacterium-mediated strains were out obvious differences in their ability, this article to all sorts of factory transfer species (Godwin et al. 1991) and a more use four root cancer agrobacterium-mediated strains (A281, GV3101, EHA105 and LBA4404) show that strain EHA105 generated transformants the highest percentage, in the Actinidia to (Wang and Lin-Wang 2007). Other factors, including the influence of Actinidia conversion efficiency contrast type, regeneration and transition conditions and Actinidia species. Meeting et al. (1991) won 26-62% of the transformation frequency use hypcotyl and 3-mm stem section from Actinidia deliciosa, incubation and Actinidia EHA101 farming. Fraser (1995) found that all test (to Actinidia Actinidia genotype and a. deliciosa) is a response to a range of tissue culture conditions and relatively easy to renewable agreement. Compared to other woody species, such as apple (James et al. 1989) and orange (Kaneyoshi et al. 1994), relatively high interest rates and regeneration of conversion got Actinidia deliciosa and Actinidia it to (Fraser et al. 1995; Janssen and Gardner 1993; meeting et al. 1991; and the king, et al. 2007). However, Actinidia lower interest rates eriantha display regeneration