端粒保护蛋白1在不同证型获得性再生障碍性贫血患者中的表达及相关性研究

摘要：目的 观察端粒保护蛋白1(POT1)在不同证型获得性再生障碍性贫血(再障)患者外周血单个核细胞的表达水平,探讨其与获得性再障及其中医辨证分型之间的关系。方法 采用实时荧光定量聚合酶链反应(RT-qPCR)检测52例不同证型获得性再障患者和20例正常对照组外周血单个核细胞中POT1 mRNA表达情况,并进行相关性分析。结果 获得性再障患者POT1 mRNA表达情况较正常对照组明显减低(P＜0.05)。肾阴虚型POT1 mRNA表达水平低于肾阳虚型,肾阴阳两虚型最低,并且与年龄有明显相关性(r＝0.374,P＝0.038)。结论 POT1参与了获得性再障的发病经过,其在外周血单个核细胞的表达水平与获得性再障的中医证型有一定相关性。

关键词：获得性再生障碍性贫血；中医证型；外周血单个核细胞；端粒保护蛋白1

DOI：10.3969/j.issn.1005-5304.2013.08.007

中图分类号：R259.565

文献标识码：A

文章编号：1005-5304(2013)08-0018-03POT1 Gene Expression in Patients of Acquired Aplastic Anemia with Different TCM Syndromes and Correlation Study WANG Yan, XU Rui-rong, CUI Si-yuan, LIU Kui, WANG Jing-yi (The Affiliated Hospital of Shandong University of TCM, Jinan 250011, China)

Abstract：Objective To observe the mRNA expression levels of POT1 in peripheral blood mononuclear cells of acquired aplastic anemia patients with different syndrome, and explore its relationship with acquired aplastic anemia and its TCM syndrome. Methods Peripheral blood mononuclear cells of 52 cases with acquired aplastic anemia and 20 cases as control group were collected to detect mRNA expression of POT1 by using real-time quantitative polymerase chain reaction (RT-qPCR), and its relation with TCM syndrome was analyzed. Results The expression levels of POT1 mRNA in patients with acquired aplastic anemia were lower significantly than control group (P＜0.05). The expression levels of POT1 mRNA in patients with deficiency of kidney-yin were lower than patients with deficiency of kidney-yang, and it was lowest in patients with kidney deficiency of both yin and yang. There was significant correlation between the expression levels of POT1 mRNA and age (r＝0.374, P＝0.038). Conclusion The changes in expression levels of POT1 play a role in the pathogenesis of acquired aplastic anemia. There is correlation between mRNA expression level of POT1 in peripheral blood mononuclear cells and TCM syndrome.

Key words：acquired aplastic anemia；TCM syndrome；peripheral blood mononuclear cells；POT1基金项目：国家科技支撑计划(2007BAI20B06)通讯作者：徐瑞荣,E-mail：

再生障碍性贫血(以下简称“再障”)根据病因的不同,可以分为获得性和遗传性的。近年研究发现,端粒、端粒酶代谢有关的酶基因缺陷及端粒相关蛋白异常也可能是导致再障发 学研究[J].江苏中医药,2008,40(7)：31.[8] 刘龙涛,吴敏,张文高,等.虎杖苷对颈动脉粥样硬化斑块稳定性的干预研究[J].北京中医药,2009,28(3)：172-175.[9] 张宪忠,杨峰,吕国庆,等.灵蒲合剂治疗颈动脉粥样硬化合并斑块的临床研究[J].中国中西医结合急救杂志,2007,14(1)：17-20.[10] 张大创,吴泽铭,陈鹏毅,等.通脉消斑Ⅰ号治疗颈动脉粥样硬化斑块临床观察[J].中国中医急症,2009,18(3)：352-353.[11] 霍清萍,梁芳,李金菩,等.稳消颗粒对兔实验性动脉粥样硬化单核细胞趋化蛋白-1及肿瘤坏死因子-α表达的影响[J].中国中医药信息杂志, 2009,16(11)：29-31.[12] 梁芳,霍清萍,王宇新,等.稳消颗粒对兔实验性动脉粥样硬化血脂代谢的影响[J].现代中西医结合杂志,2011,20(31)：3926-3927.[13] 霍清萍,梁芳,孔林,等.稳消颗粒对兔实验性动脉粥样硬化血清FIB和CRP表达的影响[J].中西医结合心脑血管病杂志,2010,8(12)：1477- 1479.(收稿日期：2013-03-28,编辑：陈静)病的重要因素之一[1-2]。再障的基本病机在于肾精亏虚,这是多数学者的共识,目前各医家基本上围绕肾阳虚型、肾阴虚型和肾阴阳两虚型开展临床及实验研究。端粒保护蛋白1(POT1)是端粒结合蛋白shelterin组分之一,对染色体的末端有直接的保护作用[3]。我们检测了获得性再障患者外周血单个核细胞的POT1基因表达水平,探讨其与再障及再障中医辨证分型之间的关系,以期为再障的诊疗提供客观依据。1 临床资料1.1 一般资料

本组观察的52例患者来自本院血液病科2011年5月－2012年1月期间收治的住院及门诊获得性再障患者。其中男性26例,女性26例,年龄7～60岁,平均34.2岁；初治患者20例,复诊患者32例；中医辨证分型中肾阳虚型18例,肾阴虚型19例,肾阴阳两虚型15例。另取健康志愿者(来自门诊健康查体人员)20例作为正常对照组,其中男性12例,女性8例,年龄20～58岁,平均年龄35.8岁。