食管鳞癌中T淋巴瘤侵袭转移诱导基因1、血管内皮生长因子和CD31的表达及其相关性研究

[摘要] 目的 检测食管鳞癌中T淋巴瘤侵袭转移诱导基因1（Tiam1）和血管内皮生长因子（VEGF）的表达特点，关注其对CD31标记的血管生成的作用。 方法 以87例食管鳞癌标本作为观察组，72例正常食管黏膜组织作为对照组，采用流式细胞技术，分别检测二组中Tiam1、VEGF和CD31的表达，探讨其在不同临床特征中的表达差别及相关性。 结果 Tiam1、VEGF和CD31蛋白在食管鳞癌中高表达，Tiam1、VEGF和CD31的表达与食管癌的浸润深度、分化程度、淋巴结转移及PCNA的增殖指数密切相关；线性相关性分析显示Tiam1、VEGF和CD31的表达之间均呈正相关性。 结论 Tiam1、VEGF和CD31在食管鳞癌中高表达，二者对肿瘤进展有重要作用，Tiam1和VEGF可能对促进CD31标记的新生血管内皮细胞的增殖有一定作用，联合检测Tiam1、VEGF和CD31蛋白可能与预后有关。

[关键词] 食管鳞癌；T淋巴瘤侵袭转移诱导基因1；血管内皮生长因子；CD31；流式细胞技术

[中图分类号] R735.1 [文献标识码] C [文章编号] 1673-7210（2012）08（b）-0095-02

The study of expression of Tiam1, VEGF and CD31 in esophageal squamous cell carcinoma and their correlations of them

WANG Qichuan WANG Qing WAN Lixin QU Zhongyu SHENG Jing

Department of Oncology Medical, the Central Hospital of Nanyang City, He′nan Province, Nanyang 473009, China

[Abstract] Objective To detect the expression characteristics of Tiam1 and VEGF in esophageal squamous cell carcinoma, and pay close attention to angiogenesis role with markers of CD31. Methods 87 cases with esophageal squamous cell carcinoma were served as observation group. 72 cases with normal esophageal mucosa were served as control group. The expressions of Tiam1, VEGF and CD31 were detected by FCM in the two groups. Their expressive relationship and different pathological characters were analyzed in the esophageal squamous cell carcinoma. Results The expression of Tiam1, VEGF and CD31 were higher in the neoplasm groups than in control group. Their expressions were correlated with invasion, differentiation, metastasis and PCNA expressions. Positive correlation were found between Tiam1, VEGF and CD31 in neoplasm by liner relationship analysis. Conclusion The higher expressions of Tiam1, VEGF and CD31 in esophageal squamous cell carcinama has the important role in tumor progression, and Tiam 1 and VEGF can promote the angiogenesis which expresses CD31 protein. The combined detection of Tiam1, VEGF and CD31 can judge the prognosis of esophageal squamous cell carcinoma.

[Key words] Esophageal squamous cell carcinoma; Tiam1; VEGF; CD31; FCM

食管鳞癌临床常见，多数预后不良，由于其是富于血管的肿瘤，因此其血管生成在肿瘤生长和侵袭中具有重要作用。血管内皮生长因子（VEGF）是血管内皮细胞的特异有丝分裂原，可以促进血管内皮细胞的增殖，促进新生血管的生成，加速肿瘤生长及转移[1]。食管鳞癌是富于血管的肿瘤，其发生发展时肿瘤细胞中VEGF的表达升高。CD31作为血管内皮的重要标志物，对新生血管内皮细胞标记的特异性强，可以有效地反应血管新生的程度。T淋巴瘤侵袭转移诱导基因1（Tiam1）是一种鸟苷酸转换因子，特异的激活与Rac相关的通路，改变肿瘤细胞形态和功能，诱导肿瘤的生长、侵袭和转移[2-3]。本研究采用流式细胞技术，检测食管鳞癌及正常食管黏膜组织中Tiam1、VEGF和CD31的表达特点，探讨Tiam1、VEGF和CD31的关系，旨在为探讨肿瘤的生物学行为提供理论支持。

1 材料与方法