大鼠咬合创伤早期牙槽骨细胞间通讯状况研究

[摘要] 目的 探讨咬合创伤早期大鼠下颌牙槽骨骨组织内细胞间的信息传递情况。方法 通过在左侧上颌第一磨牙粘接钢丝建立大鼠咬合创伤实验动物模型，24 h后局麻下分离大鼠双侧下颌牙槽骨组织，提取总RNA，进行包括27 000个基因的全功能组表达谱基因芯片检测，采用逆转录聚合酶链反应（RT-PCR）对芯片结果进行验证，对差异表达基因进行Pathway分析，找出细胞通讯相关通路。结果 芯片结果显示，差异基因共有586个，Pathway分析涉及106个不同的信号通路，其中主要涉及包括细胞间黏附分子、黏着连接、缝隙连接、焦点粘连及紧密连接在内的5个细胞间通讯相关通路，且5个通路中主要骨代谢相关细胞因子表达均显著下调。结论 咬合创伤早期大鼠牙槽骨组织内细胞间通讯受到抑制。

[关键词] 咬合创伤； 细胞间通讯； 缝隙连接； 焦点粘连； 紧密连接； 黏着连接

[中图分类号] Q 257 [文献标志码] A [doi] 10.3969/j.issn.1000-1182.2012.05.007

The intercellular communication condition of alveolar bone with traumatic occlusion at early stage in rats Wan Haoyuan1，2, Sun Huiqiang1，3, Shang Sixia1, Liu Di1，3, Li Xin1. （1. Dept. of Prosthodontics, Stomatological Hos-pital of Shandong University, Jinan 250012, China; 2. Dept. of Stomatology, The Third Affiliated Hospital of Third Military Medical University, Chongqing 400042, China; 3. Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China）

[Abstract] Objective To study the intercellular communication of alveolar bone during traumatic occlusion at early stage in rats. Methods The occlusal surface of the upper left first molar of rat was raised by placing a stainless steel wire to induce occlusal trauma in the lower left first molar. After 24 hours, the alveolar bone tissues of the lower jaws first molars at the both sides were taken out under anesthesia. The various 27 000 genes were identified with genome-wide microarray, and further were investigated with reverse transcription-polymerase chain reaction（RT-PCR） and Path-way analysis. Results Total 586 gene were found to be changed, 106 different signal pathways got involved with Path-way analysis, including cell adhesion molecules（CAMS）, adhesions junction, gap junction, focal adhesion and tight junc-tion, and the cytokines associated with bone metabolism in above 5 signal pathways were all down-regulated. Conclusion At the early phase of the occlusal trauma, intercellular communication in rat’s alveolar bone were inhibited.

[Key words] traumatic occlusion； intercellular communication； gap junction； focal adhesion； tight junction；

adhesions junction

咬合创伤是由于咬合关系不正常或咬合力量不协调，个别或某几个牙所受的咬合力量超过其牙周组织的耐受力而造成的牙周组织损伤情况，可引起牙槽骨的吸收。导致咬合创伤的常见因素有错畸形、早接触、干扰等。骨吸收是一个复杂的生物学过程，该过程中骨组织细胞间的通讯发挥重要的作用[1]，细胞间通过直接或间接信息传递，形成复杂

的通讯网络，对此通讯网络的研究将有利于进一步明确骨吸收的分子生物学机制。本实验采用人为咬合升高法建立大鼠咬合创伤模型，对咬合创伤导致牙槽骨吸收早期过程中骨组织细胞间的信息传递进行通路分析，希望进一步明确该生物过程中的细胞通讯情况。

1 材料和方法

1.1 实验动物、主要试剂和仪器

SD大鼠（山东大学实验动物中心），咬合升高粘

接复合树脂（3M公司，德国），麻醉用40 mL·kg-1水