稀释法结合LCMS/MS检测在10种中药材污染黄曲霉毒素高通量筛查中的应用研究

[摘要]该研究运用稀释法，克服了基质效应对10种中药材污染黄曲霉毒素B1，B2，G1，G2的液质联用检测的影响，高、中

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A high throughput coupled with high performance liquid chromatography-

tandem mass spectrometry method for determination of aflatoxin B1， B2，

G1， G2 in 10 traditional Chinese medicines

ZHENG Run-sheng， XU Hui*， PENG Yuan-xia， WANG Wen-li， ZHAN Ruo-ting， CHEN Wei-wen

（Research Center of Chinese Herbal Resource Science and Engineering， Key Laboratory of Chinese Medicinal Resource from

Lingnan， Ministry of Education， Guangzhou University of Chinese Medicine， Guangzhou 510006， China）

[Abstract]As the dilution procedure was applied， a simple， rapid and cost-effective high-performance liquid chromatography-tandem mass spectrometry （LC-MS/MS） method for determination of aflatoxin B1， B2， G1 and G2 was successfully by performed in a total 83 samples of 10 traditional Chinese medicines （TCMs）， which were collected from 5 different hospital pharmacies and 5 different medical stores in Guangzhou city. Matrix effects of these 10 TCMs were ranged from 80.23% to 115.5% in low， intermediate and high concentration levels， indicating that the negative effect was overcome in this study. Meanwhile， the analysis method was proved to be stable and reliable during the whole analysis using Semen Armeniacae Amarum spiked 3 concentration levels of standard solution as quality control samples and the RSD

[Key words]LC-MS/MS； traditional Chinese medicines； aflatoxins； mycotoxins

doi：10.4268/cjcmm20140222