蠲痹颗粒对胶原诱导性关节炎小鼠Th17/Treg细胞平衡调控作用的研究

【摘 要】目的：研究蠲痹颗粒对胶原诱导性小鼠关节炎Th17/Treg细胞平衡调控作用，

doi：10.3969/j.issn.2095-4174.2014.04.008

Study on the Balanced Regulation of Juanbi Granule on Th17/Treg Cells in Mice with Collagen-induced Arthritis

LIU Wei-chao，LI Ling-yu，WAN Chun-ping，LI Zhao-fu，ZHENG Xi，QI Yan，WU Jing-jin，PENG Jiang-yun

【ABSTRACT】 Objective：To study the balanced regulation of Juanbi Granule on Th17/Treg cells in mice with collagen-induced arthritis in order to explore its mechanism in the treatment of rheumatoid arthritis.Methods：The arthritis models were induced by the bovine type II collagen.20 DBA/1 mice were divided equally into the CIA group and the Juanbi granule group according to the clinical score.The arthritis clinical score method was used to observe the seriousness of the onset of arthritis in CIA mice；the flow cytometry was used to detect the subpopulation of the splenic lymphocytes （CD4，CD8，B220），and the Th17，Th1 cytokines and the expression of Treg cells in CD4+T cells.Results：Juanbi Granule could significantly reduce the clinical score of CIA mice with collagen-induced arthritis，but had no significant effect on the subpopulation of the splenic lymphocytes （CD4，CD8，B220）.Meanwhile，it down regulated the expression level of IL-17A in the CD4+T cells of CIA mice，promoting the expression of Treg cells in a certain extent.Conclusion：Regulating the balance of the Th17 cells and Treg cells and down-regulating the expression of the proinflammatory cytokine IL-17A may be one of its mechanisms in the treatment of rheumatoid arthritis.

【Keywords】 arthritis，rheumatoid；arthritis，collagen-induced；Juanbi Granule；Th17 cell；regulatory

T cells；mice

蠲痹颗粒是中医学家吴佩衡学术继承人、云南省名老中医吴生元教授基于扶阳理论总结出的治疗类风湿关节炎（rheumatoid arthritis，RA）的效方，主要由附片、川芎、赤芍、桂枝、麻黄、薏苡仁、五加皮等组成，具有温经散寒、祛风除湿、通络止痛之功，主治寒湿痹阻之RA。最新研究表明，Treg、Th17细胞功能和分化过程具有共同之处却又相互对抗，其平衡失调影响着RA发生与转归[1-2]。

1 实验材料

5 参考文献

[1] Littman DR，Rudensky AY.Th17 and regulatory T cells in mediating and restraining inflammation[J].Cell，2010，140（6）：845858.

[2] Bettelli E，Carrier Y，Gao W.Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cells[J].Nature，2006，441（7090）：235-238.

[3] 万春平，彭江云，李兆福，等.蠲痹颗粒对胶原诱导性小鼠关节炎的抑制作用及机制研究[J].中药材，2013，36（9）：1505-1507.

[4] 周茹，杨以阜，左建平.II型胶原诱导的小鼠关节炎动物模型的建立及影响因素[J].中国药理学通报，2006，22（12）：1532-1535.

[5] King C，Tangye SG， Mackay CR.T follicular helper （TFH）cells in normal and dysregulated immune responses[J].Annu Rev Immunol，2008，26：741-766.

收稿日期：2014-02-21；修回日期：2014-03-17