冰冻切片和石蜡切片对免疫组化染色效果的对比分析

时间:2022-10-08 10:21:36

冰冻切片和石蜡切片对免疫组化染色效果的对比分析

[摘要] 目的 研究皮肤、肉芽组织采用石蜡切片以及冰冻切片免疫组化的染色效果。方法 2014年8月―2016年8月期间选取Wistar大鼠共60只,双盲法随机分为A、B两组各30只。实验前对60只大鼠进行背部切割伤,脱毛后在每只大鼠背部切割伤部位取1 cm×1 cm大小的皮肤块作为标本,A组大鼠皮肤块标本采用石蜡制片,B组大鼠皮肤块标本采用冰冻制片。对两组大鼠标本切片进行免疫组化染色,观察两组大鼠标本切片染色情况。 结果 B组SP、EGF、EGFR以及FGF强阳性率分别为96.67%、100.00%、96.67%、93.33%,明显高于A组的0.00%(P

[关键词] 皮肤;肉芽组织;冰冻制片;石蜡制片;免疫组化染色

[中图分类号] R5 [文献标识码] A [文章编号] 1674-0742(2017)05(a)-0016-03

[Abstract] Objective To study the effects of paraffin sections and frozen sections on Immumohistochemical staining in the tissues of skin and granulation. Methods 60 Wistar rats were selected from August 2014 to August 2016 and randomly divided into group A and group B with 30 cases in each group respectively. Before experiment, 60 rats were concis on the back. After removing the hair, the skin with the size of 1 cm×1 cm was taken from cut injuries on the back and served as sample. The specimens of rats in group A were adopted paraffin sections, and rats in B group were adopted frozen sections. Immunohistochemical staining was performed on the two groups of rats to observe the section staining of the rats in two groups. Results The positive rates of SP, EGF, and EGFR in group B 96.67%、100.00%、96.67%、93.33% were significantly higher than those of group A 0.00%(P

[Key words] Skin; Granulation Tissue; Frozen Sections; Paraffin Sections; Immunohistochemical Staining

病理M织检查在临床上应用广泛,是众多疾病诊断的金标准。临床上常见的检查手段包括组织石蜡制片检测以及组织冰冻切片检测,两种方法的优劣在临床上众说纷纭,褒贬不一,在临床上也无相关的统一标准,争议性较大[1-2]。因此该文于2014年8月―2016年8月期间对60只大鼠模型进行研究,探讨皮肤和肉芽组织中石蜡切片和冰冻切片免疫组化染色效果的差异,为临床病理组织诊断提供理论依据,现报道如下。

[3] 姜威,尹洪涛,张安生,等.冰冻切片免疫组化在临床病理中的应用价值[J].中国现代医药杂志,2014,16(3):88-89.

[4] 罗灿峤,莫木琼,聂钊铭,等.穿刺活检组织常规石蜡和常规冰冻HE 制片的比较[J].山东医药,2014,54(41):22-24.

[5] 赵蕾,翟贺宁,王存川,等.甲状腺微小状癌的术中冰冻病理诊断分析[J].广东医学,2014,35(17):2697-2699.

[6] 许剑利.宫颈冷刀锥切术及冰冻病理检查在宫颈上皮内瘤变(CIN)Ⅲ诊断治疗中的应用分析[J].四川医学,2014,35(10):1314-1316.

[7] 孙戈.影响术中冰冻诊断正确率的临床及病理因素分析[J].医学理论与实践,2014,27(11):1508-1509.

[8] Yeh Y-C,Chou,T-Y.Pulmonary neuroendocrine tumors: Study of 90 cases focusing on clinicopathological characteri stics, immunophenotype, preoperative biopsy, and frozen section diagnoses[J].Journal of Surgical Oncology,2014,109(3):280-286.

[9] Ribback S,Flessa S,Gromoll-Bergmann K,et al.Virtual slide telepathology with scanner systems for intraoperative frozen-section consultation[J].Pathology Research and Practice,2014,210(6):377-382.

[10] Acikalin Arbil,Gumurdulu Derya,Bagir Emine Kilic,et al.The Guidance of Intraoperative Frozen Section For Staging Surgery in Endometrial Carcinoma[J].Pathology oncology research: POR,2015,21(1):119-122.

[11] 江梅珍,金海燕,何P仪,等.快速冰冻病理检查在子宫内膜癌术中的诊断价值[J].现代肿瘤医学,2015,23(16):2365-2367.

(收稿日期:2017-02-05)

上一篇:曲飞宇:终极思考催生动力! 下一篇:高校补考制度的困境和改革