脂多糖影响奶山羊肝脏代谢的1H―NMR代谢组学和形态学研究

摘 要：为研究脂多糖（lipopolysaccharide，LPS）对奶山羊肝脏营养代谢的影响，找出肝脏在LPS攻击条件下的生物标志物，探索LPS损伤肝脏的机理。该研究选择12～18月龄，体重24～28 kg的关中奶山羊15只，平均分为3组（每组5只），分别为对照组（CTL）、低剂量组（LPS-L）和高剂量组（LPS-H）。LPS-L和LPS-H分别腹腔注射20、40 μg/kgBW的LPS溶液，CTL注射等容量生理盐水。24 h后，LPS-L和LPS-H追加LPS溶液。0 h，24 h，48 h，分别从各组试验羊静脉采血，制取血清，测定代谢相关生化检测；48 h后活体采集肝脏组织，液氮保存。采用氢核磁共振（1H-nuclear magnetic resonance，1H-NMR）技术对肝脏组织各种代谢物进行检测，采用模式识别方法中的偏最小判别二乘分析法（partia1 1east squares discriminant analysis，PLS-DA），结合常规统计学中的显著性检验（t-test）对肝脏代谢物进行差异性分析。血清生化指标表明，LPS-L和LPS-H谷丙转氨酶（ALT）、总胆红素（TBIL）水平较CTL显著升高（P

关键词：代谢组学 脂多糖 肝脏 代谢物 奶山羊

Study of the Effect of Lipopolysaccharide on Hepatic Metabolism in Dairy Goat Liver Based on 1H-NMR and Morphology

Wang Linfeng Jia Shaodan Yang Gaiqing Zhu Heshui Yang Guoyu

（He'nan Agricultural University）

Abstract：In order to research the changes of dairy goat liver metabolites by using 1H-NMR test via molecular markers and explore the influence of lipopolysaccharide （LPS） on dairy goat liver.Total 15 Guanzhong dairy goats of 12-month-old with 24～28 kg live body weight （BW） were selected and divided into three groups randomly， i.e. control group， LPS-L group and LPS-H group. Animals could access to total mixed ration feeds and water ad libitum. At beginning of the experiment （0 h）， the goats in LPS-L and LPS-H groups were given 20 and 40 μg/kg BW of LPS solution via intraperitoneal injections， respectively. The goats in CTL were received same volume of saline water. 24 h later， goats in each LPS treated groups were added LPS solution once again.At the time point of 0 h， 24 h and 48 h， blood sample were drawn from neck venous and were used to extract serum after centrifuging for biochemical determination. 48 h later， the goats’ liver tissue samples were collected by biopsy and stored in liquid nitrogen. Metabolites in liver tissue were detected by 1H-NMR and analyzed by partial least squares discriminant analysis （PLS-DA） combined with t-test to identify metabolic differences under each treatment.Serum biochemical results showed the concentration of ALT and TBIL were increased significantly （P

Key Words：Metabolomics； Lipopolysaccharide； Liver； Metabolite； Dairy goat

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